Metagenomics of human microbiome: beyond 16s rDNA
نویسندگان
چکیده
منابع مشابه
Characterization of the Gut Microbiome Using 16S or Shotgun Metagenomics
The advent of next generation sequencing (NGS) has enabled investigations of the gut microbiome with unprecedented resolution and throughput. This has stimulated the development of sophisticated bioinformatics tools to analyze the massive amounts of data generated. Researchers therefore need a clear understanding of the key concepts required for the design, execution and interpretation of NGS e...
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Comparing bacterial 16S rDNA sequences to GenBank and other large public databases via BLAST often provides results of little use for identification and taxonomic assignment of the organisms of interest. The human microbiome, and in particular the oral microbiome, includes many taxa, and accurate identification of sequence data is essential for studies of these communities. For this purpose, a ...
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Although 16S rRNA gene (rDNA) sequencing is the gold standard for categorizing bacteria or characterizing microbial communities its clinical utility is limited by bias in metagenomic studies, in either the experiments or the data analyses. To evaluate the efficiency of current metagenomic methods, we sequenced seven simulated samples of ten bacterial species mixed at different concentrations. T...
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2012
ISSN: 1198-743X
DOI: 10.1111/j.1469-0691.2012.03865.x